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目录号：PF00012 概述 打印数据表 Proteintech 保证涵盖任何物种和任何应用中的 Proteintech 抗体，包括数据表中未列出的抗体。如果抗体不起作用，您可以轻松获得退款或贷记单。 了解更多 建议使用即用型流式染色缓冲液 (1X) 进行免疫荧光染色方案，使用悬浮液中的活细胞或固定细胞进行流式细胞术。流式染色缓冲液是一种含有小牛血清作为蛋白质来源的缓冲盐溶液最大限度地减少抗体和作为防腐剂的叠氮化钠 (0.09%) 的非特异性结合。该缓冲液可用于稀释抗体、细胞悬液，并可作为细胞表面染色和流式细胞术分析的洗涤步骤。 4°C 实验程序 PF00012, Flow Cytometry Staining Buffer (1X), Proteintech, IL, USA
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应用说明细胞内流式细胞术染色方案
了解不同的方法在这份\"DIY”修复和烫发指南中了解流式细胞术的细胞内染色。
Home高内涵分析![](\"https://ptglab.com/img/logos/Chromotek-part-of-%20Proteintech-logo.png\"){kind=logo}
高内涵分析高内涵筛选 (HCS) 包括多种细胞检测，以自动化、并行的方式进行，以高通量格式分析活细胞中的一组不同的生物参数。
ChromoTek 的 Chromobodies® 是荧光纳米探针，用于实时可视化活细胞内的内源性细胞内结构。使用染色体，可以实时可视化和监测动态细胞变化。这使得它们成为细胞研究的完美探针，包括高内涵分析（Schorpp et al 2016）。
染色体是源自骆驼科动物（纳米抗体或 VHH）的单域抗体，与荧光蛋白基因融合用于检测。它们在细胞内表达并与活细胞内的靶标结合。
查看所有纳米抗体以进行高内涵分析![\"HeLa](\"https://www.ptglab.com/products/pictures/ccr1.gif\")
延时视频（22 小时）：稳定表达的 HeLa 细胞的活细胞成像Cell Cycle-Chromobody (TagRFP)。
产品格式（括号内为产品引用）Actin-Chromobody®TagGFP2 (15)、TagRFP (5)Nuclear Actin-Chromobody®TagGFP2Cell Cycle-Chromobody®TagRFP (11)Dnmt1-Chromobody® TagGFP2、TagRFP (1)Histone-Chromobody®eGFP (1)Lamin-Chromobody®TagGFP2 (5)PARP1-Chromobody®TagGFP2、TagRFP (2)Vimentin-Chromobody®TagGFP (2)SupportSpectra ViewerSpectra Viewer使用我们的 Spectra Viewer 设计和计划您的实验
想要了解我们的流式细胞术和 CoraLite 抗体如何适合您的实验？我们的 Spectra Viewer 由 Fluorofinder 提供支持，可以帮助您设计和规划实验。
协议流式细胞术多色面板设计不可见文本协议用于研究细胞外和细胞内蛋白质的协议不可见文本协议实验研究细胞活力和细胞凋亡的方案不可见文本方案流式细胞术常用抗体不可见文本SupportSpectra ViewerSpectra Viewer
使用我们的 Spectra Viewer 设计和计划您的实验
想要了解我们的流式细胞术和 CoraLite 抗体如何适合您的实验？我们的 Spectra Viewer 由 Fluorofinder 提供支持，可以帮助您设计和规划实验。
协议流式细胞术多色面板设计不可见文本协议用于研究细胞外和细胞内蛋白质的协议不可见文本协议实验研究细胞活力和细胞凋亡的方案不可见文本方案流式细胞术常用抗体不可见文本研讨会癌症免疫治疗早期职业研究员会议 2022 年 4 月癌症免疫治疗早期职业研究员会议 2022 年 4 月
对不起！您要查找的页面不存在Validation Data GalleryWB analysis of HeLa using 11714-1-APHeLa cells were subjected to SDS PAGE followed by western blot with 11714-1-AP (IFITM3 antibody) at dilution of 1:5000 incubated at 4 degree celsius over night.View All Images (11)×HeLa cells were subjected to SDS PAGE followed by western blot with 11714-1-AP (IFITM3 antibody) at dilution of 1:5000 incubated at 4 degree celsius over night.IHC staining of human lung cancer using 11714-1-APImmunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11714-1-AP (IFITM3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).View All Images (11)×Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11714-1-AP (IFITM3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC staining of human lung cancer using 11714-1-APImmunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11714-1-AP (IFITM3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).View All Images (11)×Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11714-1-AP (IFITM3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP experiment of HeLa using 11714-1-APIP Result of anti-IFITM3 (IP:11714-1-AP, 3ug; Detection:11714-1-AP 1:1500) with HeLa cells lysate 2400ug.View All Images (11)×IP Result of anti-IFITM3 (IP:11714-1-AP, 3ug; Detection:11714-1-AP 1:1500) with HeLa cells lysate 2400ug.IF Staining of HeLa using 11714-1-APImmunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11714-1-AP (IFITM3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).View All Images (11)×Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11714-1-AP (IFITM3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).FC experiment of HeLa using 11714-1-AP1X10^6 HeLa cells were stained with 0.2ug IFITM3 antibody (11714-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.View All Images (11)×1X10^6 HeLa cells were stained with 0.2ug IFITM3 antibody (11714-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. -/ - Cat No. 11714-1-AP Freight/Packing: - Country/Region Size/Concentration Quantity Add to Basket Add to Quote Please visit your regions distributor: Proteintech Guarantee Request bulk or custom quote Chat with a team member The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note. Learn More Positive WB detected inHeLa cells, mouse colon tissue, HepG2 cellsPositive IP detected inHeLa cellsPositive IHC detected inhuman lung cancer tissue, human colon cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0Positive IF detected inHeLa cellsPositive FC detected inHeLa cells Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody. Recommended dilutionApplicationDilutionWestern Blot (WB)WB : 1:2000-1:10000Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WBImmunohistochemistry (IHC)IHC : 1:50-1:500Immunofluorescence (IF)IF : 1:50-1:500Sample-dependent, check data in validation data gallery 11714-1-AP targets IFITM3 in WB, IP, IHC, IF, FC, Blocking assay,ELISA applications and shows reactivity with human, mouse, rat samples. Calculated molecular weight 133 aa, 15 kDaObserved molecular weight 14 kDaGenBank accession numberBC006794 Gene symbol IFITM3 Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Background InformationIFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. Catalog#11714-1-AP is a rabbit polyclonal antibody raised against the full-length of human IFITM3. ProtocolsProduct Specific ProtocolsWB protocol for IFITM3 antibody 11714-1-APDownload protocolIHC protocol for IFITM3 antibody 11714-1-APDownload protocolIF protocol for IFITM3 antibody 11714-1-APDownload protocolIP protocol for IFITM3 antibody 11714-1-APDownload protocolFC protocol for IFITM3 antibody 11714-1-APDownload protocolStandard ProtocolsClick here to view our Standard Protocols PublicationsAll (78)KD/KO (24)WB (61)IHC (5)IF (14)IP (2)FC (7)SpeciesApplicationTitlehumanWB,IFNat Chem BiolIFITM3 directly engages and shuttles incoming virus particles to lysosomes.Authors - Jennifer S SpenceKO ValidatedView ArticlehumanWBInt J Biol MacromolConstruction, expression and antiviral activity analysis of recombinant adenovirus expressing human IFITM3 in vitro.Authors - Shouwen DuView ArticlehumanWBMBioVpr Enhances HIV-1 Env Processing and Virion Infectivity in Macrophages by Modulating TET2-Dependent IFITM3 Expression.Authors - Qi WangKD ValidatedView ArticlemouseIHCCell RepCell Lineage Tracing Identifies Hormone-Regulated and Wnt-Responsive Vaginal Epithelial Stem Cells.Authors - Ayesha AliView ArticlehumanWBCell Chem BiolSite-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase.Authors - Xiaojun WuKO ValidatedView ArticlehumanFCbioRxivOpposing activities of IFITM proteins in SARS-CoV-2 infection.Authors - Guoli ShiView Article See more citations Related ProductsIFITM2 Rabbit Polyclonal antibody12769-1-APIFITM1-Specific Mouse Monoclonal antibody60074-1-IgIFITM2-specific Mouse Monoclonal antibody66137-1-IgOsteopontin Rabbit Polyclonal antibody22952-1-APNAMPT/PBEF Rabbit Polyclonal antibody11776-1-APIFITM1 Rabbit Polyclonal antibody11727-3-APIFN Gamma Rabbit Polyclonal antibody15365-1-AP WB FiguresWB analysis of HeLa using 11714-1-APHeLa cells were subjected to SDS PAGE followed by western blot with 11714-1-AP (IFITM3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.WB analysis of HeLa using 11714-1-APHeLa cells were subjected to SDS PAGE followed by western blot with 11714-1-AP (IFITM3 antibody) at dilution of 1:5000 incubated at 4 degree celsius over night.WB analysis of mouse colon using 11714-1-APmouse colon tissue were subjected to SDS PAGE followed by western blot with 11714-1-AP (IFITM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.IHC FiguresIHC staining of human colon cancer using 11714-1-APImmunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11714-1-AP (IFITM3 antibody) at dilution of 1:200 (under 10x lens).IHC staining of human colon cancer using 11714-1-APImmunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11714-1-AP (IFITM3 antibody) at dilution of 1:200 (under 40x lens).IHC staining of human lung cancer using 11714-1-APImmunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11714-1-AP (IFITM3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC staining of human lung cancer using 11714-1-APImmunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11714-1-AP (IFITM3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC staining of human lung cancer using 11714-1-APImmunohistochemical analysis of paraffin-embedded human lung cancer using 11714-1-AP (IFITM3 antibody) at dilution of 1:100 (under 10x lens).IP FiguresIP experiment of HeLa using 11714-1-APIP Result of anti-IFITM3 (IP:11714-1-AP, 3ug; Detection:11714-1-AP 1:1500) with HeLa cells lysate 2400ug.IF FiguresIF Staining of HeLa using 11714-1-APImmunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11714-1-AP (IFITM3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).FC FiguresFC experiment of HeLa using 11714-1-AP1X10^6 HeLa cells were stained with 0.2ug IFITM3 antibody (11714-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. \"IFITM3 antibodies\" comparison At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs. We have selected the top cited antibodies from these vendors for you to compare. WB : 1:2000-1:10000IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WBIHC : 1:50-1:500IF : 1:50-1:500
