ProteinTech/CDK6 Polyclonal antibody/150ul/14052-1-AP,ProteinTech,,ProteinTech,ptglab,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：ProteinTech/CDK6 Polyclonal antibody/150ul/14052-1-AP

产品编号：14052-1-AP

市场价：¥1980.00

场地：美国(厂家直采)

产品分类：蛋白类>多肽>多肽合成>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$99.00

品牌： ProteinTech

公司：Proteintech Group, Inc

公司分类：

商品介绍

Tested Applications

Positive WB detected in	Jurkat cells, HeLa cells,C2C12 cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	human lung cancer tissue, human endometrial cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 1 publications below
WB	See 66 publications below
IHC	See 5 publications below
IF	See 1 publications below

Product Information

14052-1-AP targets CDK6 inWB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CDK6 fusion protein Ag5275
Full Name	cyclin-dependent kinase 6
Calculated molecular weight	326 aa, 36 kDa
Observed molecular weight	36-40 kDa
GenBank accession number	BC027989
Gene symbol	CDK6
Gene ID (NCBI)	1021
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20^oC storage.

Background Information

Cyclin-dependentkinase6(CDK6)isanenzymebelongingtotheCDKfamily.CDK6isacatalyticsubunitofa

proteinkinasecomplexthatisessentialforprogressionthroughtheG1phaseofthecellcycleandG1/Stransition,

thusactivatingcellproliferation.CDK6partnerswithcyclinstophosphorylatetheretinoblastoma(pRb)proteinto

releaseitsbindingpartnerE2F;atranscriptionfactorthatdrivesgeneexpressionnecessaryforDNAreplication

(PMID:8114739).CDK6waslongthoughtofasaredundanthomologofCDK4astheyperformsimilarrolesincell

cycleprogression.ButrecentlyithasbecomeapparentthatCDK6andCDK4differintissue-specificfunctions,

whereCDK6playsaroleindifferentiation(PMID:16410727)butalsoincontributiontotumordevelopment.

WhatisthemolecularweightofCDK6?

ThemolecularweightofCDK6isapproximately36–40kDa(PMID:23563707,8114739).

WhatisthesubcellularlocalizationofCDK6?

CDK6isubiquitouslyexpressedthroughthenucleusandcytoplasm.However,themostactivecomplexesare

locatedinthenucleiofproliferatingcells.

WhatistheexpressionpatternofCDK6?

CDK6activityfirstoccursmidwaythroughtheG1phase.ItsexpressionisregulatedbyD-typecyclinsand

membersoftheINK4familyofCDKinhibitors(PMID:7739547).

WhatisCDK6’sroleincancer?

CDK6isinvolvedinacriticalregulatorypointinthecellcycleandsoisfoundtobeunbalancedinapproximately

80–90%oftumors(PMID:23356980).Lossofcellcyclecontrolistheinitialstepinthedevelopmentofthe

hallmarksofcancer,suchassustainedproliferativesignalinganddysregulatedcellularenergetics.Deregulation

ofCDK6hasanimportantroleinlymphomabyincreasingangiogenesis(PMID:23948297)andupregulatedCDK6

expressionisalsoassociatedwithapoorclinicalprognosisinmedulloblastoma(PMID:23172372).

Protocols

Product Specific Protocols
WB protocol for CDK6 antibody 14052-1-AP	Download protocol
IHC protocol for CDK6 antibody 14052-1-AP	Download protocol
IF protocol for CDK6 antibody 14052-1-AP	Download protocol
IP protocol for CDK6 antibody 14052-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

品牌介绍

Proteintech是一家以研发、生产、销售抗体/重组蛋白及相关产品为主要业务的生物技术公司。拥有严格的抗体生产、质检质控体系，产品涵盖整个生命科学领域。Proteintech作为全球最大的专业抗体生产商之一，凭借一支锐意进取的技术团队和稳定的技术平台，已研发和生产出14000多种特种抗体和14000多种人源蛋白产品。2018年4月正式收购首家人源细胞生产人类蛋白的Humanzyme公司，新增Humankine高活性人源蛋白产品系列。目前，Proteintech已建立全球市场销售网络，美国、欧洲、中国和日本均有现货库存，全球24小时现货发售。