RNASEH1 Antibody 15606-1-AP | Proteintech-ProteinTech公司新闻-蚂蚁淘厂家直采,部分现货.

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亲和纯化

亲和纯化是一种从复杂混合物中富集或纯化蛋白质的技术生物分子，如细胞裂解液、细胞培养上清液或血清等。感兴趣的蛋白质选择性地被配体（如抗体或纳米抗体）结合，配体附着在基质（如琼脂糖珠）上。

通常，目的蛋白质通过重组表达并与蛋白质或肽标签融合，该标签通过亲和树脂特异性且可逆地结合。富集的蛋白质可以通过使用肽等分子竞争与亲和树脂结合的竞争性洗脱来释放，也可以简单地通过改变缓冲液条件（例如 pH 值变化或离子强度变化）来释放。

最常见，亲和纯化可以批量纯化或通过填充有亲和树脂的柱进行（亲和层析)。

纳米抗体特别适合亲和纯化，因为它们结合了高选择性和高度稳健性。由于纳米抗体非常稳定，即使在极端缓冲条件下也能保持功能，因此可以应用高度严格的洗涤方案（例如去污剂、低 pH 值、高盐等）和亲和树脂的多个再生循环。因此，它们是亲和纯化的完美工具。

查看用于蛋白质纯化的 Spot-Cap™

点标记蛋白质的纯化。在 4°C 下用 Spot-peptide 洗脱。

Synonyms H1RNA, Ribonuclease H type II, ribonuclease H1, RNase H1, RNASEH1, RNH1 Validation Data GalleryWB analysis of HeLa using 15606-1-APHeLa cells were subjected to SDS PAGE followed by western blot with 15606-1-AP (RNASEH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.View All Images (4)×HeLa cells were subjected to SDS PAGE followed by western blot with 15606-1-AP (RNASEH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.IP experiment of HeLa using 15606-1-APIP Result of anti-RNASEH1 (IP:15606-1-AP, 3ug; Detection:15606-1-AP 1:500) with HeLa cells lysate 1200ug.View All Images (4)×IP Result of anti-RNASEH1 (IP:15606-1-AP, 3ug; Detection:15606-1-AP 1:500) with HeLa cells lysate 1200ug.IF Staining of HeLa using 15606-1-APImmunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15606-1-AP (RNASEH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).View All Images (4)×Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15606-1-AP (RNASEH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). -/ - Cat No. 15606-1-AP Freight/Packing: - Country/Region Size/Concentration Quantity Add to Basket Add to Quote Please visit your regions distributor: Proteintech Guarantee Request bulk or custom quote Chat with a team member The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note. Learn More Positive WB detected inHeLa cells, human brain tissuePositive IP detected inHeLa cellsPositive IF detected inHeLa cells Recommended dilutionApplicationDilutionWestern Blot (WB)WB : 1:500-1:2400Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WBImmunofluorescence (IF)IF : 1:50-1:500Sample-dependent, check data in validation data gallery 15606-1-AP targets RNASEH1 in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples. Calculated molecular weight 32 kDaObserved molecular weight 32 kDaGenBank accession numberBC002973 Gene symbol RNASEH1 Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Background InformationRibonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196]. ProtocolsProduct Specific ProtocolsWB protocol for RNASEH1 antibody 15606-1-APDownload protocolIF protocol for RNASEH1 antibody 15606-1-APDownload protocolIP protocol for RNASEH1 antibody 15606-1-APDownload protocolStandard ProtocolsClick here to view our Standard Protocols PublicationsAll (25)KD/KO (3)WB (16)IF (11)SpeciesApplicationTitlehumanWB,IFNucleic Acids ResBRCA1-associated R-loop affects transcription and differentiation in breast luminal epithelial cells.Authors - Huai-Chin ChiangView ArticlemouseWB,IFF1000ResFormation of 53BP1 foci and ATM activation under oxidative stress is facilitated by RNA:DNA hybrids and loss of ATM-53BP1 expression promotes photoreceptor cell survival in mice.Authors - Vaibhav BhatiaView ArticlehumanIFMol Cancer ResThe antitumor drugs trabectedin and lurbinectedin induce transcription-dependent replication stress and genome instability.Authors - Emanuela TuminiView ArticlehumanWBBiochem Biophys Res CommunXRN2 is required for the degradation of target RNAs by RNase H1-dependent antisense oligonucleotides.Authors - Shin-Ichiro HoriView ArticlehumanIFMol Cell BiolMutual Balance of Histone Deacetylases 1 and 2 and the Acetyl Reader ATAD2 Regulates the Level of Acetylation of Histone H4 on Nascent Chromatin of Human Cells.Authors - Pavlo LazarchukView ArticlehumanWBSci RepFANCM suppresses DNA replication stress at ALT telomeres by disrupting TERRA R-loops.Authors - Xiaolei PanView Article See more citations ReviewsAdd a reviewThe reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.FHHuai-Chin (Verified Customer) (06-08-2019)Very good antibody. I\'ve tried it in Western blot, cultured cell immunofluorescence, and tissue IHC. All works well.Applications: Western Blot,Immunohistochemistry,Immunofluorescence,Primary Antibody Dilution: 1:3000 for Western, 1:500 for IHC, 1:200 for IF-ICCell Tissue Type: Mammary gland/ MCF7 WB FiguresWB analysis of HeLa using 15606-1-APHeLa cells were subjected to SDS PAGE followed by western blot with 15606-1-AP (RNASEH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.WB analysis of human brain using 15606-1-APhuman brain tissue were subjected to SDS PAGE followed by western blot with 15606-1-AP (RNASEH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.IP FiguresIP experiment of HeLa using 15606-1-APIP Result of anti-RNASEH1 (IP:15606-1-AP, 3ug; Detection:15606-1-AP 1:500) with HeLa cells lysate 1200ug.IF FiguresIF Staining of HeLa using 15606-1-APImmunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15606-1-AP (RNASEH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). \"RNASEH1 antibodies\" comparison At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs. We have selected the top cited antibodies from these vendors for you to compare. WB : 1:500-1:2400IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WBIF : 1:50-1:500