ProteinTech/RB1 Polyclonal antibody/150ul/10048-2-Ig,ProteinTech,,ProteinTech,ptglab,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：ProteinTech/RB1 Polyclonal antibody/150ul/10048-2-Ig

产品编号：10048-2-Ig

市场价：¥1980.00

场地：美国(厂家直采)

产品分类：蛋白类>多肽>多肽合成>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$99.00

品牌： ProteinTech

公司：Proteintech Group, Inc

公司分类：

商品介绍

Tested Applications

Positive WB detected in	Jurkat cells,A431 cells
Positive IP detected in	A431 cells
Positive IHC detected in	human prostate cancer tissue,human lung cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:600-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)	IHC : 1:50-1:200
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 3 publications below
WB	See 23 publications below
IHC	See 5 publications below
IF	See 3 publications below

Product Information

10048-2-Ig targets RB1 inWB, IP, IHC, IF, ELISA applications and shows reactivity with human,mouse samples.

Tested Reactivity	human,mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RB1 fusion protein Ag0006
Full Name	retinoblastoma 1
Calculated molecular weight	110 kDa
Observed molecular weight	110 kDa
GenBank accession number	BC040540
Gene symbol	RB1
Gene ID (NCBI)	5925
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20^oC storage.

Background Information

RB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. It is a key regulator of entry into cell division that acts as a tumor suppressor. RB1 acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. RB1 controls histone H4 "Lys-20" trimethylation and inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1"s activity. This antibody is a rabbit polyclonal antibody raised against human RB1 fusion protein.

Protocols

Product Specific Protocols
WB protocol for RB1 antibody 10048-2-Ig	Download protocol
IHC protocol for RB1 antibody 10048-2-Ig	Download protocol
IP protocol for RB1 antibody 10048-2-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

品牌介绍

Proteintech是一家以研发、生产、销售抗体/重组蛋白及相关产品为主要业务的生物技术公司。拥有严格的抗体生产、质检质控体系，产品涵盖整个生命科学领域。Proteintech作为全球最大的专业抗体生产商之一，凭借一支锐意进取的技术团队和稳定的技术平台，已研发和生产出14000多种特种抗体和14000多种人源蛋白产品。2018年4月正式收购首家人源细胞生产人类蛋白的Humanzyme公司，新增Humankine高活性人源蛋白产品系列。目前，Proteintech已建立全球市场销售网络，美国、欧洲、中国和日本均有现货库存，全球24小时现货发售。