ProteinTech/pan Ras Monoclonal antibody/150ul/60309-1-Ig,ProteinTech,,ProteinTech,ptglab,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：ProteinTech/pan Ras Monoclonal antibody/150ul/60309-1-Ig

产品编号：60309-1-Ig

市场价：¥1980.00

场地：美国(厂家直采)

产品分类：蛋白类>多肽>多肽合成>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$99.00

品牌： ProteinTech

公司：Proteintech Group, Inc

公司分类：

商品介绍

Tested Applications

Positive WB detected in	fetal human brain tissue, HEK-293 cells,Neuro-2a cells,ROS1728 cells,human brain tissue,rat brain tissue,mouse brain tissue,pig brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human breast cancer tissue,human colon cancer tissue,human lung cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:20000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 1 publications below
WB	See 5 publications below
IHC	See 3 publications below

Product Information

60309-1-Ig targets pan Ras inWB, IP, IHC, FC, ELISA applications and shows reactivity with human,mouse,rat,pig samples.

Tested Reactivity	human,mouse,rat,pig
Cited Reactivity	human, swine
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	pan Ras fusion protein Ag2700
Full Name	v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated molecular weight	188 aa, 21 kDa
Observed molecular weight	21 kDa
GenBank accession number	BC013572
Gene symbol	KRAS
Gene ID (NCBI)	3845
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20^oC storage.

Background Information

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.

Protocols

Product Specific Protocols
WB protocol for pan Ras antibody 60309-1-Ig	Download protocol
IHC protocol for pan Ras antibody 60309-1-Ig	Download protocol
IP protocol for pan Ras antibody 60309-1-Ig	Download protocol
FC protocol for pan Ras antibody 60309-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

品牌介绍

Proteintech是一家以研发、生产、销售抗体/重组蛋白及相关产品为主要业务的生物技术公司。拥有严格的抗体生产、质检质控体系，产品涵盖整个生命科学领域。Proteintech作为全球最大的专业抗体生产商之一，凭借一支锐意进取的技术团队和稳定的技术平台，已研发和生产出14000多种特种抗体和14000多种人源蛋白产品。2018年4月正式收购首家人源细胞生产人类蛋白的Humanzyme公司，新增Humankine高活性人源蛋白产品系列。目前，Proteintech已建立全球市场销售网络，美国、欧洲、中国和日本均有现货库存，全球24小时现货发售。