ProteinTech/Caspase 6/p18/p11 Polyclonal antibody/150ul/10198-1-AP,ProteinTech,,ProteinTech,ptglab,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：ProteinTech/Caspase 6/p18/p11 Polyclonal antibody/150ul/10198-1-AP

产品编号：10198-1-AP

市场价：¥1980.00

场地：美国(厂家直采)

产品分类：蛋白类>多肽>多肽合成>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$99.00

品牌： ProteinTech

公司：Proteintech Group, Inc

公司分类：

商品介绍

Tested Applications

Positive WB detected in	Staurosporine treated Jurkat cells, mouse brain tissue,Jurkat cells
Positive IHC detected in	human prostate cancer tissue, human lung cancer tissue,human skin tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 1 publications below
WB	See 5 publications below

Product Information

10198-1-AP targets Caspase 6/p18/p11 inWB, IHC, ELISA applications and shows reactivity with human,mouse,rat samples.

Tested Reactivity	human,mouse,rat
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Caspase 6/p18/p11 fusion protein Ag0257
Full Name	caspase 6, apoptosis-related cysteine peptidase
Calculated molecular weight	33 kDa, 22 kDa
Observed molecular weight	33-35 kDa
GenBank accession number	BC000305
Gene symbol	CASP6
Gene ID (NCBI)	839
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20^oC storage.

Background Information

Caspase-6 belongs to caspase family of cysteinyl-aspartate specific proteases.Precursor of CASP6 produces two subunits, large (18kDa) and small (16kDa) that dimerize. It cleaves poly(ADP-ribose) polymerase, as well as lamins and is involved in the activation cascade of caspases responsible for apoptosis execution. Researches showed that CASP6 could be an early instigator of neuronal dysfunction and regulates B cell activation and differentiation into plasma cells by modifying cell cycle entry. IRAK3 is an important target for CASP6. It can reveal five bands of 28, 32, 36, 49, and 64 kDa in human neurons and fetal brain in western blot, the 32 and 28 kDa bands represent procaspase-6 and pro-arm caspase-6. Procaspase-6 is more abundant than pro-arm caspase-6 in adult tissue, whereas pro-arm caspase-6 is more abundant than pro-caspase-6 in fetal brain and cultured neurons. The higher molecular mass bands at 49 and 64 kDa likely represent dimers of p28 and p32.(PMID:10438520). In rat testis, it can be detected two bands of 34 kDa and 12 kDa or 14 kDa(PMID:12538628).

Protocols

Product Specific Protocols
WB protocol for Caspase 6/p18/p11 antibody 10198-1-AP	Download protocol
IHC protocol for Caspase 6/p18/p11 antibody 10198-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

品牌介绍

Proteintech是一家以研发、生产、销售抗体/重组蛋白及相关产品为主要业务的生物技术公司。拥有严格的抗体生产、质检质控体系，产品涵盖整个生命科学领域。Proteintech作为全球最大的专业抗体生产商之一，凭借一支锐意进取的技术团队和稳定的技术平台，已研发和生产出14000多种特种抗体和14000多种人源蛋白产品。2018年4月正式收购首家人源细胞生产人类蛋白的Humanzyme公司，新增Humankine高活性人源蛋白产品系列。目前，Proteintech已建立全球市场销售网络，美国、欧洲、中国和日本均有现货库存，全球24小时现货发售。