ProteinTech/PKC Alpha Polyclonal antibody/150ul/21991-1-AP,ProteinTech,,ProteinTech,ptglab,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：ProteinTech/PKC Alpha Polyclonal antibody/150ul/21991-1-AP

产品编号：21991-1-AP

市场价：¥1980.00

场地：美国(厂家直采)

产品分类：蛋白类>多肽>多肽合成>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$99.00

品牌： ProteinTech

公司：Proteintech Group, Inc

公司分类：

商品介绍

Tested Applications

Positive WB detected in	Jurkat cells,HEK-293 cells,NIH/3T3 cells, rat kidney tissue,human peripheral blood platelets
Positive IP detected in	NIH/3T3 cells
Positive IHC detected in	human thyroid cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in	HeLa cells,NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)	IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 1 publications below
WB	See 11 publications below
IHC	See 1 publications below
IF	See 3 publications below

Product Information

The immunogen of 21991-1-AP is PKC Alpha Fusion Protein expressed in E. coli.

Tested Reactivity	human,mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PKC Alpha fusion protein Ag17275
Full Name	protein kinase C, alpha
Calculated molecular weight	77 kDa
Observed molecular weight	77 kDa
GenBank accession number	AK055431
Gene symbol	PRKCA
Gene ID (NCBI)	5578
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20^oC storage.

Background Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCA is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

Protocols

Product Specific Protocols
WB protocol for PKC Alpha antibody 21991-1-AP	Download protocol
IHC protocol for PKC Alpha antibody 21991-1-AP	Download protocol
IF protocol for PKC Alpha antibody 21991-1-AP	Download protocol
IP protocol for PKC Alpha antibody 21991-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

品牌介绍

Proteintech是一家以研发、生产、销售抗体/重组蛋白及相关产品为主要业务的生物技术公司。拥有严格的抗体生产、质检质控体系，产品涵盖整个生命科学领域。Proteintech作为全球最大的专业抗体生产商之一，凭借一支锐意进取的技术团队和稳定的技术平台，已研发和生产出14000多种特种抗体和14000多种人源蛋白产品。2018年4月正式收购首家人源细胞生产人类蛋白的Humanzyme公司，新增Humankine高活性人源蛋白产品系列。目前，Proteintech已建立全球市场销售网络，美国、欧洲、中国和日本均有现货库存，全球24小时现货发售。